Manual of Practical Biochemistry, 3rd Edition

M D Rafi

ISBN: 9789389211658 | Year: 2020 | Paperback | Pages: 296 | Language : English

Book Size: 180 X 240 mm | Territorial Rights: World

Price: 350.00

About the Book

This book introduces some of the most widely used experimental procedures in biochemistry for students of MBBS and other allied sciences. In this new edition, all the chapters have been thoroughly revised to conform to the Competency-Based Medical Education (CBME) curriculum.

Salient features
• A wide range of qualitative analysis and quantitative estimations and measurements in biochemistry explained with emphasis on principles, procedures, observations and clinical interpretations

• Theory of the underlying principles and procedures included so that students are able to appreciate the biochemical basis of the demonstrated experiments/techniques

• Covers all the competencies prescribed in the CBME curriculum

• Several new topics or expanded treatment of topics such as electrophoresis, chromatography, estimation of pH and preparation of buffers, biomedical waste management, isolation and quantification of DNA, and biochemical tests for common clinical conditions included in this edition

• Features viva questions commonly encountered in practical examinations

• Includes MCQs, problem-based exercises, questions relating to interpretation of laboratory data and case studies to aid students in their preparations for competitive examinations

Contributors (Author(s), Editor(s), Translator(s), Illustrator(s) etc.)

Rafi MD, Dean and Medical Director of Surabhi Institute of Medical Sciences, Siddipet, obtained his MBBS degree from Kakatiya Medical College, Warangal, and MD from Gandhi Medical College, Hyderabad. He is well-known for his passion for maximising the learning experience for his students through innovative teaching methods. Dr Rafi has several research publications to his credit in journals of repute.

Table of Contents

Preface to the Third Edition
Preface to the First Edition

QUALITATIVE EXPERIMENTS
 
1. Safety in the Laboratory 

2. Reactions of Carbohydrates 

Reactions of Glucose 
Reactions of Fructose
Reactions of Maltose 
Reactions of Lactose 
Reactions of Sucrose 
Identification of Unknown Carbohydrate 

3. Reactions of Lipids 

Reactions of Triacylglycerols 
Reactions of Cholesterol 

4. Reactions of Proteins 

Precipitation Reactions of Proteins 
Colour Reactions of Proteins 
Reactions of Albumin 
Reactions of Casein 
Reactions of Gelatin
Reactions of Peptone 
Identification of Unknown Protein 

5. Reactions of Non-protein Nitrogenous (NPN) Substances 

Urea 
Uric Acid 
Creatinine 
Identification of Unknown NPN Substance

6. Identification of Unknown Substance of Biochemical Importance 
Carbohydrates, Proteins and NPN Substances 

7. Analysis of Urine 

Physical Examination 
Microscopic Examination 
Chemical Analysis 

8. Analysis of Abnormal Constituents in Urine 

Glucose 
Ketone Bodies 
Proteins 
Blood 
Bile Salts 
Bile Pigments 
Identification of Unknown Abnormal Constituents in Urine 

9. Q
ualitative Analysis of Saliva 

10. Qualitative Analysis of Gastric Juice 


11. Qualitative Analysis of Bile 


12. Qualitative Analysis of Milk 


13. Food Ana
lysis 

QUANTITATIVE EXPERIMENTS 


14. Introduction to Clinical Biochemistry 

Glassware and Instruments Used in the Laboratory 
Solutions 
Sample Collection and Handling 
Colorimetry 
ABG Analysis 
Immunodiffusion 
Quality Control 

15. 
Blood Glucose 

16. 
Glucose Tolerance Test 

17. Blood Urea and BUN 


18. Serum and Urinary Creatinine 


19. Uric Acid 


20. Serum Total Proteins and Albumin–Globulin Ratio 


21. Cholesterol and Lipid Profile 


22. Bilirubin 


23. Aminotransferases (AST and ALT or SGOT and SGPT) 


24. Alkaline and Acid Phosphatases (ALP and ACP) 


25. Creatine Kinase and Its Isoenzymes 


26. Amylase and Lipase 


27. Calcium and Phosphorus 


28. Serum Electrolytes 


29. Quantitative Analysis of 
CSF 

30. Electrophoresis 

Paper Electrophoresis 
Gel Electrophoresis 
Isoelectric Focussing 
Capillary Electrophoresis 

31. Chromatography 

Thin Layer Chromatography (TLC) 
Gas–Liquid Chromatography 
Ion Exchange Chromatography 
Gel Exclusion (Permeation) Chromatography 
Affinity Chromatography 
High Performance (Pressure) Liquid Chromatography (HPLC) 

32. Spectroscopic Examination of Hemoglobin and Its Derivatives 


33. Radioimmunoassay (RIA) and Enzyme-linked Immunosorbent Assay (ELISA) 


34. Estimation of pH and Preparation of Buffers 


35. Biomedical Waste Management 


36. DNA Isolation and Quantification 


37. Biochemica
l Tests for Common Clinical Conditions 

APPENDICES

  1. Practical Biochemistry Question Bank
  2. Multiple-choice Questions (MCQs) in Practical Biochemistry
  3. III. Problem-solving Exercises, Interpretation of Laboratory Data and Case Studies in Practical Biochemistry
  4. IV. Test Procedures for Qualitative and Quantitative Experiments in a Nutshell for Ready Reference

Index

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