This book introduces some of the most widely used experimental procedures in biochemistry for students of MBBS and other allied sciences. In this new edition, all the chapters have been thoroughly revised to conform to the Competency-Based Medical Education (CBME) curriculum.Salient features• A wide range of qualitative analysis and quantitative estimations and measurements in biochemistry explained with emphasis on principles, procedures, observations and clinical interpretations
• Theory of the underlying principles and procedures included so that students are able to appreciate the biochemical basis of the demonstrated experiments/techniques
• Covers all the competencies prescribed in the CBME curriculum
• Several new topics or expanded treatment of topics such as electrophoresis, chromatography, estimation of pH and preparation of buffers, biomedical waste management, isolation and quantification of DNA, and biochemical tests for common clinical conditions included in this edition
• Features viva questions commonly encountered in practical examinations
• Includes MCQs, problem-based exercises, questions relating to interpretation of laboratory data and case studies to aid students in their preparations for competitive examinations
Rafi MD, Dean and Medical Director of Surabhi Institute of Medical Sciences, Siddipet, obtained his MBBS degree from Kakatiya Medical College, Warangal, and MD from Gandhi Medical College, Hyderabad. He is well-known for his passion for maximising the learning experience for his students through innovative teaching methods. Dr Rafi has several research publications to his credit in journals of repute.
Preface to the Third EditionPreface to the First EditionQUALITATIVE EXPERIMENTS 1. Safety in the Laboratory 2. Reactions of Carbohydrates Reactions of Glucose Reactions of FructoseReactions of Maltose Reactions of Lactose Reactions of Sucrose Identification of Unknown Carbohydrate 3. Reactions of Lipids Reactions of Triacylglycerols Reactions of Cholesterol 4. Reactions of Proteins Precipitation Reactions of Proteins Colour Reactions of Proteins Reactions of Albumin Reactions of Casein Reactions of GelatinReactions of Peptone Identification of Unknown Protein 5. Reactions of Non-protein Nitrogenous (NPN) Substances Urea Uric Acid Creatinine Identification of Unknown NPN Substance
6. Identification of Unknown Substance of Biochemical Importance Carbohydrates, Proteins and NPN Substances 7. Analysis of Urine Physical Examination Microscopic Examination Chemical Analysis 8. Analysis of Abnormal Constituents in Urine Glucose Ketone Bodies Proteins Blood Bile Salts Bile Pigments Identification of Unknown Abnormal Constituents in Urine 9. Qualitative Analysis of Saliva 10. Qualitative Analysis of Gastric Juice 11. Qualitative Analysis of Bile 12. Qualitative Analysis of Milk 13. Food Analysis QUANTITATIVE EXPERIMENTS 14. Introduction to Clinical Biochemistry Glassware and Instruments Used in the Laboratory Solutions Sample Collection and Handling Colorimetry ABG Analysis Immunodiffusion Quality Control 15. Blood Glucose 16. Glucose Tolerance Test 17. Blood Urea and BUN 18. Serum and Urinary Creatinine 19. Uric Acid 20. Serum Total Proteins and Albumin–Globulin Ratio 21. Cholesterol and Lipid Profile 22. Bilirubin 23. Aminotransferases (AST and ALT or SGOT and SGPT) 24. Alkaline and Acid Phosphatases (ALP and ACP) 25. Creatine Kinase and Its Isoenzymes 26. Amylase and Lipase 27. Calcium and Phosphorus 28. Serum Electrolytes 29. Quantitative Analysis of CSF 30. Electrophoresis Paper Electrophoresis Gel Electrophoresis Isoelectric Focussing Capillary Electrophoresis 31. Chromatography Thin Layer Chromatography (TLC) Gas–Liquid Chromatography Ion Exchange Chromatography Gel Exclusion (Permeation) Chromatography Affinity Chromatography High Performance (Pressure) Liquid Chromatography (HPLC) 32. Spectroscopic Examination of Hemoglobin and Its Derivatives 33. Radioimmunoassay (RIA) and Enzyme-linked Immunosorbent Assay (ELISA) 34. Estimation of pH and Preparation of Buffers 35. Biomedical Waste Management 36. DNA Isolation and Quantification 37. Biochemical Tests for Common Clinical Conditions APPENDICES
Index