Table of Contents

Introduction | Designing the tissue culture laboratory | Equipping the tissue culture laboratory | Glassware and plasticware used for tissue culture | Aseptic techniques | Sterilisation of materials to be used for tissue culture | Tissue culture media | Types of tissue culture | Organ and organotypic cultures | Primary cultures | Transformation, differentiation and dedifferentiation | Culture of cell lines | Protocols for routine characterisation of cell lines | Specialised techniques in tissue culture | Stem cells cultures | Autoradiography of cultured cells | Cytogenic analysis of cultured cells | Flow cytometric analysis of lymphocytes and cultured cells | Hybridoma cultures to generate monoclonal antibodies | Laser confocal microcsopy and its application in cell biology | Primary culture of chick embryo fibroblasts | Troubleshooting.

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